# TP (Two Photon)-alpha [Catalog # P026]

**■ Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/223813_XNt1qxZox0NSw4uBWX?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/223828_jJljxIRPhoAyGjI15F?q=80&s=1280x180&t=outside&f=webp)

**TP-alpha **(Two Photon alpha cell probe) was discovered from “two photon green fluorescence library” by cell-based screening. **TP-alpha** is a pancreatic alpha cell selective probe over beta cell or acinar cell. TP-alpha showed dose dependent fluorescence increase upon treatment of glucagon, with low response to insulin or other proteins.

**■ Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/223852_I3BqKOTKT3iyD3GfBW?q=80&s=1280x180&t=outside&f=webp)

Antibody confirmation of TP-α selectivity by imaging and flow-cytometry. (A) Cultured dissociated islets stained with 1 μM TPα (in green) followed by immunostaining with glucagon antibody (in red). Merged image demonstrates the TP-α stained cells to be glucagon positive alpha cells (white arrow). Scale bar is 50 μm. (B) Dual parametric dot plot for TP-α and glucagon antibody. About 17 000 islet cell counted.

■ **Related probes**: Glucagon yellow (GY)

■ **Known Property**
pancreatic alpha cell
■ **Application**
Immunofluorescence and 3D imaging of live alpha cells using two photon microscopy
■ **Cell selectivity mechanism**
POLD (glucagon) 

■ **Reference**

1. **Glucagon-secreting alpha cell selective two-photon fluorescent probe TP-α: for live pancreatic islet imaging**, Agrawalla, B. K.; Chandran, Y.; Phue, W. H.; Lee, S. C.; Jeong, Y. M.; Wan, S. Y.; Kang, N. Y._; Chang, Y. T._ J. Am. Chem. Soc. 2015, 137, 5355-5362.

■ **ORDER**

**International**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

-  For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■ **Data sheet PDF file**

TP alpha data sheet v0.2.pdf

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