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CO-1 [Catalog # P049]
■ Chemical structure & Information
CO-1 is cell permeable background-free probe through optimized lipophilicity, water solubility and charged van der Waals surface area. CO-1, a cyclooctyne-containing BODIPY probe, is also specifically label intracellular azide-tagged organelles & engineered proteins with minimum background.
■ Cell Image
Fluorescence imaging of mitochondria, lysosome and golgi apparatus in U-2 OS cells labelled with CO-1. Cells pre-treated with TPP-Az, Morph-Az or Sphingo-Az were incubated with 2 μM CO-1 in growth media at 37 °C for 1 h and followed by counterstaining with organelle trackers. Scale bar, 15 μm. (iv) Fluorescence imaging of histone H2B in live U-2 OS. U-2 OS cells were co-transfected with plasmids pIre-Azi3 and pmH2B-6-mKate2-16tag to incorporate UAA Azi into H2B-mKate2 at site 16. After Azi incorporation, cells were labelled with 10 μM CO-1 for 90 min at 37 °C. Cells were then washed and imaged. The mKate2 (red) signals were detected in cell nuclei only, and colocalized with the CO-1 (green) signals in the merged image. Scale bar, 5 μm.
■ Related probes: AzG-1
■ Known Property
Bio-orthogonal Click reaction
■ Application
Immunofluorescence
■ Cell selectivity mechanism
unknown
Bio-orthogonal Click reaction
■ Application
Immunofluorescence
■ Cell selectivity mechanism
unknown
■ Reference
1.
Development of background-free tame fluorescent probes for intracellular live cell imaging, Alamudi, S. H.; Satapathy, R.; Kim, J.; Su, D.; Ren, H.; Das, R.; Hu, L.; Alvarado-Martínez, E.; Lee, J. Y.; Hoppmann, C.; Peña-Cabrera, E.; Ha, H. H.; Park, H. S.; Wang, L.; Chang, Y. T.* Nat. Commun. 2016, 7, 11964.
■ ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equivalent to 1 mM / 100 µL)
■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C
■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
precipitated out from buffer solution.
■ Data sheet PDF file
CO-1 data sheet v0.2.pdf709.66KB