# F22 [Catalog # P023]

**■ Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/220438_LVWKzaHYSPP3tu4ceG?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/220449_XfQDDItSTucyJZ9QQX?q=80&s=1280x180&t=outside&f=webp)

**F22** is a selective RNA probe over DNA. **F22** was disclosed through in vitro DNA/RNA selectivity screening and live cell staining for nucleolus

**■ Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/220509_rIZE8IZGLvOgLFySGm?q=80&s=1280x180&t=outside&f=webp)

Live Cell RNA Staining with Selected Dyes E36, E144, and **F22** were tested at a 5 mM concentration. The picture of F22-stained 3T3 cells was obtained in a 1 mM dye concentration.
1000x magnification was utilized in the imaging. The scale bar represents 5 mm. Image brightness and contrast were slightly adjusted to improve picture quality. E36, E144 (green: FITC channel), and F22 (red: Cy3 channel) are shown.

■** Related probes**: E36, F22

■ **Known Property**
RNA probe
■ **Application**
Selective fluorescent imaging of nuclear structure in live cells
■ **Cell selectivity mechanism**
RNA

■ **Reference**

1. **RNA-selective, live cell imaging probes for studying nuclear structure and function**., Li, Q., Kim, Y. K., Namm, J., Kulkarni, A., Rosania, G., Ahn, Y. H., Chang, Y. T.* Chem. Biol. 2006, 13, 615-623.

2. **A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36**, E144 and F22 Li, Q.; Chang, Y. T.* Nat. Protoc. 2006, 1, 2922-2932.

3. **RNA buffers the phase separation behavior of prion-like RNA binding proteins**, Maharana, S.; Wang, J.; Papadopoulos, D. K.; Richter, D.; Pozniakovsky, A.; Poser, I.; Bickle, M.; Rizk, S.; Guillén-Boixet, J.; Franzmann, T.; Jahnel, M.; Marrone, L.; Chang, Y. T.; Sterneckert, J.; Tomancak, P.; Hyman, A. A.; Alberti, S. Science 2018, 360, 918-921.

4. **RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation**, Guillén-Boixet, J.; Kopach, A.; Holehouse, A. S.; Wittmann, S.; Jahnel, M.; Schlüßler, R.; Kim, K.; Trussina, I. R. E. A.; Wang, J.; Mateju, D.; Poser, I.; Maharana, S.; Ruer-Gruß, M.; Richter, D.; Zhang, X.; Chang, Y. T.; Guck, J.; Honigmann, A.; Mahamid, J.;
4. Hyman, A. A.; Pappu, R. V.; Alberti, S.; Franzmann, T. M. Cell 2020, 181, 346-361.

■ **ORDER**

**International**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

-  For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■ **Data sheet PDF file**

F22 data sheet v0.2.pdf

For the site tree, see the [root Markdown](https://senprobe.kr/.md).