Check out our newly launched website!
Go to homepage
CDb8 [Catalog # P008]
■ Chemical structure & Information
CDb8 (Compound of Designation blue 8) is a xanthone based blue fluorescent probe for mouse embryonic stem cell (mES). CDb8 stains the outside of mES colony, i.e. glycocalyx area [1]. Glycocalyx is enriched with glycoproteins and glycolipids. The co-staining with related ES probes showed that CDy1 stains inside ES colony, and CDg4 and CDb8 stains the surface of ES colony [2]. Based on the imaging data, the binding target of CDb8 may be glycogen in glycocalyx.
■ Cell Image
Selective staining of mESC by CDb8. (a) Chemical structure of CDb8; (b) mESC was selectively stained by CDb8 at 1 mM for 1 h. Upper panel: mouse embryonic fibroblasts (MEF), middle: mouse embryonic stem cells (mESC), lower panel: mESC on MEF feeder. (c) Flow cytometry analysis of DMSO control cells. (d) Flow cytometry analysis of CDb8 stained cells. The cells are loaded after 1 h incubation at 1 mM. B.F: bright field, scale bar: 100 mm.
■ Related probes: CDy1, CDg4, CDy9
■ Known Property
mouse embryonic stem cell (mES) probe
mouse embryonic stem cell (mES) probe
■ Application
Immunofluorescenec
Immunofluorescenec
■ Cell selectivity mechanism
COLD (maybe glycogen)
COLD (maybe glycogen)
■ Reference
1.
Solid phase combinatorial synthesis of xanthone library using click chemistry and its application to embryonic stem cell probe, Ghosh, K. K; Ha, H. H.; Kang, N. Y.; Chandran, Y.; Chang, Y. T.* Chem. Commun. 2011, 47, 7488-7490
2.
Development of fluorescent Chalcone library and its application in the discovery of a mouse embryonic stem cell probe, Lee, S. C.; Kang, N. Y.; Park, S. J.; Yun, S. W.; Chandran, Y.; Chang, Y. T.* Chem. Commun. 2012, 48, 6681-6683.
■ ORDER
International:
USD 350 + VAT (10%) + Fedex delivery charge
In Korea:
KRW 450,000 + VAT (10%)
100 nmol (dried form: equivalent to 1 mM / 100 µL)
■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C
■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
precipitated out from buffer solution.
■ Data sheet PDF file
CDb8 data sheet v0.2.pdf611.25KB