# Histamine Blue [Catalog # P060]

**■ Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250509/191337_Y3lM8Y0dH3LNDdUEjp?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250509/191406_5GsUSfktyKmwuj7pKq?q=80&s=1280x180&t=outside&f=webp)

**Histamine Blue** is a mesoionic acid fluoride showing a 14-fold fluorescence increase upon condensation with histamine. **Histamine Blue** displays very good selectivity over a broad range of signalling molecules and metabolites due to the catalytic effect of the imidazole group, and is the first fluorescent small molecule for imaging histamine in live RBL-2H3 basophils. **Histamine Blue** can also monitor the uptake and de novo synthesis of histamine in live RAW 264.7 macrophages and its fluorescence intensity correlates well with the histamine levels in cell extracts.

**■ Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250509/191511_z0xbxahDhRPJn98bso?q=80&s=1280x180&t=outside&f=webp)

A) Reaction of 4a with histamine in PBS. Fluorescence spectra of 4a before (black) and after (blue) reaction with histamine; exc: 340 nm. Fluorescent images of PBS solutions of 4a and 5i under a 365 nm lamp.

B) RBL-2H3 basophils after incubation with 20µM **Histamine Blue**. (a) Bright field image, (b) DRAQ5 nuclear counterstaining (TXR), (c) Histamine Blue staining (DAPI), (d) merged a, b and c. Scale bar: 20 mm.

![Image](https://upload.cafenono.com/image/slashpagePost/20250509/191609_zpbAEDHOn4BzIvWopD?q=80&s=1280x180&t=outside&f=webp)

Three-color imaging for granulocytes. a) Schematic of granulocytes sorted by Fluorescence-Activated Cell Sorting (FACS). b) Three-color imaging of granulocytes. The sorted granulocytes were stained NeutropG (0.2 μM), **Histamine Blue** (10 μM), and APC anti-Siglec-8 for 1 h.

■ **Known Property**
Basophil selective through histamine sensing
■ **Application**
Immunofluorescence
■ **Cell selectivity mechanism**
Unknown

■ **Reference
**

1. **Imaging Histamine in Live Basophils and Macrophages with a Fluorescent Mesoionic Acid Fluoride**, Kielland, N.; Vendrell, M.; Lavilla, R._; Chang, Y. T._ Chem. Commun. 2012, 48, 7401-7403

2. **Neutrophil Selective Fluorescent Probe Development through Metabolism-Oriented Live-cell Distinction**, Gao, M.; Lee, S. H.; Park, S. H.; Ciaramicoli, L. M.; Kwon, H. Y.; Cho, H.; Jeong, J.; Chang, Y. T.* Angew. Chem. Int. Ed. Engl. 2021, 60, 23742-23749.

■ **ORDER**

**International**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

-  For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■ **Data sheet PDF file**

Histamine blue data sheet.pdf

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